The PDF file includes:
- Fig. S1. Phage display yields human Fabs with high affinity and specificity for mouse
erythrocytes. - Fig. S2. No significant differences between transcripts of naïve and OVAlow.
- Fig. S3. Synthesis of an irrelevant-Fab-OVA to control for size and expression vector
of antigen. - Fig. S4. A8B1 persists on erythrocytes and is capable of stimulating T cells for days
after initial dosing. - Fig. S5. T cell dysfunction is not due to bolus of antigen presentation immediately
after injection. - Fig. S6. Characterization of the Batf3-deficient mouse.
- Fig. S7. Flow cytometry gating strategy for OT-I and OT-II cells.
- Fig. S8. Flow cytometry gating strategy for inhibitory, exhaustion, and anergy receptors.
- Fig. S9. Flow cytometry gating strategy for tetramer staining.
Other Supplementary Material for this manuscript includes the following:
- Table S1. Differentially expressed genes between A8B1, OVAhigh, OVAlow, and naïve (Excel file).
- Table S2. Ingenuity pathway analysis of A8B1 versus OVAhigh (Excel file).
- Table S3. Amino acid sequences for Fab and antigen (Excel file).
- Table S4. List of antibodies for flow cytometry (Excel file).
- Table S5. Raw data (Excel file).
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